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    Worthington 动物脂肪/脂肪组织分离指南

    发布者:艾美捷科技    发布时间:2022-05-28     
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    组织分离/原代细胞分离,是酶在组织培养研究和细胞生物学研究中的主要应用。尽管多年来在这些应用中广泛使用酶,但它们在解离和收获中的作用机制尚不清楚。

     tissue-worthington.png

    研究人员在科学文献中搜索有关理想酶和组织解离的最佳条件的信息时,经常会遇到相互矛盾的数据。大部分变化源于细胞外基质的复杂性和动态性,以及在细胞分离应用中使用相对粗糙、不确定的酶制剂。

    本指南总结了Worthington如何使用这些酶来完成对应动物脂肪/脂肪组织的解离,以及原代细胞采集的常规操作。 本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南


    动物脂肪/脂肪 
    种属种属详情细胞名称酶组合推荐,含推荐浓度培养基参考文献
    禽类

    淀粉样蛋白原纤维

    Amyloid Fibrils

    Collagenase Type 3: 0.02%;
    Deoxyribonuclease I: 0.001%
    50mM TrisScherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
    禽类
    淀粉样蛋白原纤维 / Amyloid FibrilsCollagenase Type 3: 0.02%;
    Deoxyribonuclease I: 0.001%
    50mM TrisScherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016
    Bear, Ursus arctos脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%HBSSGehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes., Cytotechnology 68, 2177-91, 2016
    Bovine脂肪细胞 / AdipocytesCollagenase Type 1: 40 u/mlKrebs-Ringer bicarbonateYang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue, J Dairy Sci 56, 350, 1973
    犬/狗23-33 kg基质细胞血管 / Stromal vascularCollagenase: see referenceMedia 199EWilliams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013
    犬/狗Canine肾脂肪来源的细胞 / Renal adipose derived cellsCollagenase Type 1: 0.3%DMEMBasu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
    犬/狗Canine, 20-25 kg脂肪干细胞 / Adipose stem cellsCollagenase: see referenceMedia-199Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009
    犬/狗Canine白色脂肪 / White fatCollagenase: 0.05%Kreb's Ringer bicarbonate bufferDiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
    Equine脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%PBSVidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008
    Equine, 1-5 year脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%PBSVidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007
    Fish, Atlantic salmon前脂肪细胞 / PreadipocytesCollagenase Type 1: 0.1%HBSSTodorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids., Biochim Biophys Acta 1781, 326, 2008
    沙鼠Gerbil of unknown age (also rat, hamster, rabbit, lamb, guinea-pig)棕色脂肪 / Brown fatCollagenase Type 1: 0.10%Bicarbonate bufferNedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
    豚鼠Guinea-pig, adult (also rat, hamster, gerbil, rabbit, lamb)棕色脂肪 / Brown fatCollagenase Type 1: 0.10%Bicarbonate bufferNedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
    仓鼠Hamster, adult(also rat, gerbil, rabbit, lamb, guinea-pig)棕色脂肪 / Brown fatCollagenase Type 1: 0.10%Bicarbonate bufferNedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982
    仓鼠Hamster, 5 week-12 month白色脂肪 / White fatCollagenase: 0.05%Kreb's Ringer bicarbonate bufferDiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
    Human脂肪来源的干细胞 / Adipose derived stemCollagenase Type 4: 150-300 u/mlRPMI-1640Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019
    Human脂肪细胞 / AdipocytesCollagenase Type 2: 0.01-0.5%DMEMTsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad., J Oral Sci , , 2018
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%HBSSWahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity, Nature 541, 81, 2017
    Human基质细胞 / StromalCollagenase Type 2: 0.075%DMEMDuscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells., Cytotherapy 19, 1491-1500, 2017
    Human间充质基质细胞 / Mesenchymal stromalCollagenase Type 1: 0.075%PBSLin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage, Cytotherapy 19, 1426-1437, 2017
    Human间充质干细胞 / Mesenchymal stemCollagenase Type 2: 0.2%DMEM/F-12Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner., Stem Cells 35, 1636-1646, 2017
    Human脂肪来源的间充质干细胞 / Adipose derived mesenchymal stemCollagenase animal free: 0.1%PBSClark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017
    Human脂肪来源的内皮 / Adipose derived endothelialCollagenase Type 1: 0.1%DMEMSzoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue., Cytotherapy 18, 242-52, 2016
    Human脂肪干细胞 / Adipose stemCollagenase Type 1: 0.1%DMEM/F12Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016
    Human牙髓干细胞 / Dental pulp stemCollagenase animal free: 0.1%HBSSHu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice., Stem Cell Res Ther 7, 130, 2016
    Human间充质基质细胞 / Mesenchymal stromalCollagenase Type 4: 0.2%PBSChoudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015
    Human脂肪干细胞 / Adipose derived stem cellsCollagenase Type 2: 0.1%HBSSSatish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015
    Human, female脂肪基质细胞 / Adipose stromalCollagenase Type 1: 0.075%DMEMKinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers., Stem Cells Transl Med 4, 146, 2015
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%PBSSeaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
    Human脂肪基质细胞-血管 / Adipose stroma-vascularNeutral Protease: 2.4 u/ml;
    Collagenase: 250 u/ml
    PBSEsteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells., Stem Cells 33, 1277-91, 2015
    Human, adult脂肪来源的间充质干细胞 / Adipose derived mesenchymal stemCollagenase Type 2: 0.1%see referenceAl-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014
    Human, adult脂肪来源的干细胞 / Adipose derived stemCollagenase Type 1: 0.15%DMEMKoellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014
    Human间充质基质细胞 / Mesenchymal stromalCollagenase Type 1: 0.1%PBSNajar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy 16, 1220-8, 2014
    Human脂肪基质细胞干细胞 / Adipose stromal stemCollagenase animal free: 200 u/mlDMEM/Hams F-12Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng 19, 473-8, 2013
    Human基质细胞血管部分 / Stromal vascular fractionCollagenase Type 1: 0.075%PBSDoi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med 7, 864, 2013
    Human脂肪来源的干细胞 / Adipose-derived stemCollagenase Type 1: 0.1%PBSCervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1, 206-20, 2012
    Human血管周围干细胞 / Perivascular stemCollagenase Type 2: 0.1%DMEMJames, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1, 673, 2012
    Human脂肪来源的基质细胞血管 / Adipose derived stromal vascularCollagenase Type 1: 0.1%PBSGentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1, 230-6, 2012
    Human基质细胞血管,脂肪细胞 / Stromal vascular, adipocytesCollagenase Type 1: 0.1%DMEMGentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction., Stem Cells Transl Med 1, 341-51, 2012
    Human, adult基质细胞血管 / StromovascularCollagenase Type 1: 0.1%PBS with BSAHagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing., J Immunol Methods 386, 50, 2012
    Human脂肪来源的基质细胞 / Adipose derived stromalCollagenase Type 1: 0.1%PBSNaaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications., Cell Tissue Res 348, 119, 2012
    Human脂肪来源的干细胞 / Adipose derived stemCollagenase Type 1: 0.1%DMEM/F-12Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction., Plast Reconstr Surg 129, 1247, 2012
    Human, male 40-60 years脂肪干细胞 / Adipose derived stem cellsCollagenase: 0.25%;
    Deoxyribonuclease I: 0.002%
    PBSBlasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell 3, 5, 2011
    Human肾脂肪来源的细胞 / Renal adipose derived cellsCollagenase Type 1: 0.3%DMEMBasu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
    Human脂肪来源的间充质 / Adipose derived mesenchymalCollagenase Type 1: 0.1%L-DMEMYang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci 18, 59, 2011
    Human脂肪来源的干细胞 / Adipose derived stemCollagenase Type 1: 0.1%DMEM/Ham's F-12Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702, 17-27, 2011
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%RPMIBasu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation., Obesity 19, 476, 2011
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%KRBPeters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture., PLoS ONE 5, e15689, 2010
    Human脂肪组织来源的干细胞 / Adipose tissue-derived stemCollagenase Type 2: 1.0%DMEM/F12Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92, 979, 2010
    Human基质细胞血管 / Stromal vascularCollagenase Type 2: 0.1%HBSSZimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue., Cytometry 77, 22-30, 2010
    Human脂肪基质细胞 / Adipose stromalCollagenase Type 1: 0.1%DMEM/Hams F-12Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy 12, 538, 2010
    Human脂肪细胞 / AdipocytesCollagenase: 0.075%DMEMSuga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism., Stem Cells 27, 238, 2009
    Human脂肪基质细胞 / Adipose stromalCollagenase Type 1: 0.1%DMEMCai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27, 230, 2009
    Human脂肪组织来源的干细胞 / Adipose tissue-derived stemCollagenase Type 2: 1.0%DMEM/F12Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering., Biomaterials 30, 6844, 2009
    Human脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%DMEMTandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation., Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009
    Human脂肪来源的基质细胞 / Adipose derived stromal cellsCollagenase Type 1: see referenceDMEMTraktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks, Circ Res 102, 77, 2008
    Human基质细胞 / StromalCollagenase Type 1: 0.2%PBSMinana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue., Stem Cells 26, 2696, 2008
    Human基质细胞 / StromalCollagenase Type 1: 0.1%;
    Neutral Protease:
    DMEMNie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells., Stem Cells 26, 2735, 2008
    Human干细胞 / StemCollagenase: 280 u/mlD-PBSPilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med 3, 705-15, 2008
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%HBSSBujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat, J Endocrinol 192, 279-88, 2007
    Human脂肪来源的基质细胞 / Adipose derived stromal cellsCollagenase Type 1: 0.15%DMEMSchaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007
    Human脂肪干细胞 / Adipose derived stem cellsCollagenase Type 1: 0.1%DMEM/F12Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212, 702-9, 2007
    Human间充质干细胞 / Mesenchymal stemCollagenase Type 1: 0.1%HBSSJeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006
    Human前脂肪细胞 / PreadipocytesCollagenase Type 1: 196 u/mlM199Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes., Stem Cells 24, 1218-25, 2006
    Human脂肪细胞,基质细胞血管 / Adipocytes, stromal vascularCollagenase: 0.2%HBSSBoquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005
    Human多能脂肪来源的干细胞 / Multipotent adipose derived stemCollagenase: 0.2%DMEMRodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J Exp Med 201, 1397-405, 2005
    Human成体干细胞 / Adult stem cellsCollagenase Type 1: 0.1%PBSDevireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005
    Human, adult, obese脂肪细胞 / AdipocytesCollagenase Type 2: 0.1%DMEM/F12Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation, J Clin Endocrinol Metab 89(10), 4833, 2004
    Human, adult, female前脂肪细胞 / PreadipocytesCollagenase Type 1: 0.2%DMEM/F-12Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5, J Endocrinol 183, 331, 2004
    Human, adult脂肪细胞 / AdipocytesCollagenase Type 1: 0.13%see referenceFain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans, Endocrinology 145, 2273, 2004
    Human基质细胞血管,脂肪细胞 / Stromal vascular, adipocytesCollagenase: 0.2%DMEM/F12Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
    Human基质细胞血管,脂肪细胞,干细胞 / Stromal vascular, adipocytes, stemCollagenase: 300 u/mlPBSMiranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004
    Human, adult, female, non-obese脂肪细胞 / AdipocytesCollagenase Type 2: 0.05%DMEMGesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression, Horm Metab Res 35, 158, 2003
    Human脂肪细胞 / AdipocytesCollagenase Type 1: see referenceSalinePatwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery, Ped Neurosurg 36, 314, 2002
    Human, adult, male and female脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%DMEM/F12McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences, J Clin Endocrinol Metab 87, 1327, 2002
    Human处理过的脂肪抽吸细胞 / Processed lipoaspirate cellsCollagenase Type 1: 0.075%PBSZuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002
    Human脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%HBSSMcTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue, Int J Obes Relat Metab Disord 24, 875-81, 2000
    Human, adult, male and female脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%DMEM/Ham's F-12Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1, Eur J Endocrinol 141 (4), 436, 1999
    Human, adult, male and female脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%DMEM/F12Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake, J Clin Endocrinol Metab 84, 2550, 1999
    Human, non-diabetic, male脂肪细胞 / AdipocytesCollagenase Type 1: 0.05%Kreb's Ringer bicarbonate bufferAnderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes, Diabetologia 13, 589, 1977
    小鼠Mouse脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%DMEMJones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet., Sci Rep 10, 2380, 2020
    小鼠Mouse脂肪基质细胞 / Adipose stromalCollagenase Type 1: 0.1%HBSSBowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis., Stem Cells 35, 532-544, 2017
    小鼠Mouse脂肪间质基质细胞 / Adipose mesenchyal stromalCollagenase Type 2: 0.2%DMEMMaria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy 18, 1129-45, 2016
    小鼠Mouse脂肪基质细胞 / Adipose stromalCollagenase Type 1: 0.1%PBSYao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference., Stem Cells 34, 2587-2600, 2016
    小鼠Mouse脂肪细胞 / AdipocytesCollagenase: 0.1%KrebsJang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life., Endocrinology 156, 3737, 2015
    小鼠Mouse脂肪细胞 / AdipocytesCollagenase Type 1: 0.1%PBSSeaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015
    小鼠Mouse, 20 week基质细胞血管 / Stromal vascularCollagenase Type 2: 0.2%RPMI-1640Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice., Exp Anim 62, 319, 2013
    小鼠Mouse脂肪来源的干细胞 / Adipose derived stemCollagenase Type 2: 0.1%DMEMTakahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1, 803, 2012
    小鼠Mouse干细胞和祖 / Stem and progenitorCollagenase Type 2: 0.2%HBSSHan, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010
    小鼠Mouse, 3 week脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%HBSSDe Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells., Stem Cells 27, 2761, 2009
    小鼠Mouse白色脂肪细胞 / White adipocytesCollagenase Type 2: 0.1%DMEMWong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins., Am J Physiol/Endo 296, 820, 2009
    小鼠Mouse脂肪基质细胞 / Adipose stromalCollagenase Type 2: 0.075%DMEMThangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia., Stem Cells 27, 266, 2009
    小鼠Mouse脂肪组织来源的干细胞 / Adipose tissue-derived stemCollagenase Type 1: 0.075%Modified EaglesCho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27, 259-65, 2009
    小鼠Mouse, C57Bl/6J脂肪细胞 / AdipocytesCollagenase Type 1: 0.15%DMEM/F12Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005
    小鼠Mouse, 3-6 day and 2-3 month脂肪来源的基质细胞 / Adipose-derived stromal cellsCollagenase Type 2: 0.075%PBSCowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects, Nat Biotechnol 22, 560-7, 2004
    小鼠Mouse基质细胞血管,脂肪细胞 / Stromal vascular, adipocytesCollagenase: 0.2%DMEM/F12Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004
    小鼠Mouse, C57BL/6J and FVB, male, 8-9 week脂肪细胞 / AdipocytesCollagenase: see referenceDMEMRuan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes, J Biol Chem 278, 47585, 2003
    小鼠Mouse, both sexes脂肪细胞 / AdipocytesCollagenase: 0.05%Krebs-Ringer Phosphate HEPES (KRPH)Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice, Am J Physiol/Endo 281, E1249, 2001
    小鼠Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male血管内皮 / Vascular endothelialCollagenase: 0.2%PBSLaunder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue, J Immunol Methods 102, 45, 1987
    Porcine, female, <1 year脂肪间充质干细胞 / Adipose mesenchymal stemCollagenase Type 1: 0.1%DMEMWilliams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008
    Porcine, crossbred, male 1-4 day脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%DMEM/F12Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes, J Anim Sci 81, 3008, 2003
    Porcine, crossbred脂肪细胞 / AdipocytesCollagenase Type 1: 0.067%Krebs-RingerLiang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues., J Anim Sci 80, 963-70, 2002
    Porcine, 8-9 week脂肪 / AdiposeCollagenase Type 1: 300 u/mlHEPESDing, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo, Comp Biochem Physiol B 123, 307, 1999
    Porcine, neonatal脂肪细胞 / AdipocytesCollagenase Type 1: 0.3%Krebs-Ringer bicarbonate albuminWang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine, J Nutr 129, 139-45, 1999
    Porcine, crossbred, 1-3 day脂肪,基质细胞血管 / Adipose, Stromal-vascularCollagenase Type 1: 0.2%DMEM/F12Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture, J Anim Sci 75, 105, 1997
    大鼠Rat, SD, 2-3 month间充质基质细胞 / Mesenchymal stromalCollagenase Type 1: 0.1%DMEMDayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy 19, 937-946, 2017
    大鼠Rat, 9 week脂肪基质细胞 / Adipose stromalCollagenase: 0.1%DMEMOhta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy 19, 839-848, 2017
    大鼠Rat间充质干细胞 / Mesenchymal stemCollagenase Type 1: 0.075%DMEMEmre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy 17, 543-59, 2015
    大鼠Rat, SD, 5 month间充质基质细胞 / Mesenchymal stromalCollagenase Type 2: 0.075%DMEMVeronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy 17, 215-23, 2015
    大鼠Rat脂肪细胞 / AdipocytesCollagenase Type 1: 0.3%Krebs-RingerOgasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes., Biochem Biophys Res Commun 464, 348-53, 2015
    大鼠Rat, adult基质细胞血管 / Stromal vascularCollagenase Type 1: 0.2%PBSLeblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct., Stem Cells Transl Med 2, 896-905, 2013
    大鼠Rat, male, 300g脂肪细胞 / AdipocytesCollagenase Type 2: 0.33%RPMI 1640Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue., J Lipid Res 53, 2797, 2012
    大鼠Rat, Lewis, male肾脂肪来源的细胞 / Renal adipose derived cellsCollagenase Type 1: 0.3%DMEMBasu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011
    大鼠Rat, SD, neonatal棕色脂肪细胞 / Brown adipocytesCollagenase Type 4: 0.1%;
    Neutral Protease: 0.1%;
    Trypsin: 0.05%
    PBSLiu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol 2010, 104296, 2010
    大鼠Rat脂肪来源的干细胞 / Adipose derived stemCollagenase Type 1: 0.1%DMEMWei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27, 478, 2009
    大鼠Rat脂肪组织来源的干细胞 / Adipose tissue-derived stemCollagenase Type 2: 0.075%DMEMTomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue., Stem Cells 26, 330, 2008
    大鼠Rat, Wistar, 4 week脂肪细胞 / AdipocytesCollagenase: 0.2%Ham's F12Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro, Cell Struct Funct 28, 55, 2003
    大鼠Rat, SD脂肪细胞 / AdipocytesCollagenase Type 1: 0.2%KRHBMora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency, Endocrinology 142, 1999, 2001
    大鼠Rat, SD, male, 4-7 weeks棕色脂肪细胞 / Brown adipocytesDeoxyribonuclease I: 0.5%DMEMOmatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes, J Physiol 521 (3), 601, 1999
    大鼠Rat脂肪细胞 / AdipocytesCollagenase Type 2: 0.2%DMEM /F-12Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995
    大鼠Rat, SD, male, 130-160 g脂肪,附睾脂肪垫 / Adipose,Epididymal fat padsCollagenase: 0.3%Kreb's-Ringer bicarbonate buffer modifiedCharron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo, J Biol Chem 265, 7994, 1990
    大鼠Rat, 3 day前脂肪细胞 / PreadipocytesCollagenase Type 3: 0.10%Parker Medium 199Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture, Biochim Biophys Acta 968, 231, 1988
    大鼠Rat, SD, various weights and ages棕色脂肪细胞,肩胛间和宫颈贮库 / Brown adipocytes,Interscapular & cervical depotsCollagenase: 0.2%;
    Soybean Trypsin Inhibitor: 0.3%
    Krebs Ringer bicarbonate bufferWoodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline, Biochem J 238, 395, 1986
    大鼠Rat, CD, male, 150-200 g脂肪细胞,附睾脂肪垫 / Adipocytes,Epididymal fat padsCollagenase: 0.1%Krebs Ringer bicarbonate bufferPessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ?-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes, J Biol Chem 258, 7386, 1983
    大鼠Rat, Wistar, albino, male, 100-140 g脂肪细胞,附睾脂肪垫 / Adipocytes,Epididymal-fat padsCollagenase: 0.3%Kreb's RingerGreen, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites, Biochem J 180, 365, 1979
    大鼠Rat, Fischer, 344, male, 9 - 13 week白色脂肪 / White fatCollagenase: 0.3%Kreb's Ringer bicarbonate bufferStiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot, Am J Physiol 229, 1561, 1975
    大鼠Rat, Wistar, male, 5 week-16 month白色脂肪 / White fatCollagenase: 0.05%Kreb's Ringer bicarbonate bufferDiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971
    大鼠Rat (CFE), albino, female棕色脂肪 / Brown fatCollagenase Type 1: 0.10%Bicarbonate bufferFain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells, J Biol Chem 242, 1887, 1967
    大鼠Rat, SD, male, 160-210 g胖的 / FatCollagenase: 0.3%Albumin-bicarbonate bufferRodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis, J Biol Chem 239 (2), 375, 1964
    松鼠Squirrel棕色脂肪细胞 / Brown adipocytesCollagenase Type 2: 0.16%Krebs-Ringer phosphateMcFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator., Am J Physiol Regul Integr Comp Physiol 312, R434-R442, 2017


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